KMID : 0984720060380020085
|
|
Infection and Chemotherapy 2006 Volume.38 No. 2 p.85 ~ p.90
|
|
In Vitro Activities of Daptomycin Against Recent Clinical Isolates of Methicillin-resistant Staphylococci and Vancomycin-resistant Enterococci in Korea
|
|
Lee Hyuk-Min
Park Seo-Jin Choi Young-Sun Park Doe-Young Roh Kyoung-Ho Yum Jong-Hwa Yong Dong-Eun Lee Kyung-Won Chong Yun-Sop
|
|
Abstract
|
|
|
Background: Daptomycin is a novel cyclic lipopeptide antibiotic that exhibits in vitro bactericidal activity against gram-positive pathogens including methicillin-resistant staphylococci and vancomycin-resistant enterococci. The aim of this study is to determine the in vitro activities of daptomycin against recent clinical isolates of methicillin-resistant staphylococci and vancomycin-resistant enterococci in Korea.
Materials & Methods: A total of 117 clinical strains of methicillin-resistant staphylococci and vancomycin-resistant enterococci were isolated at a tertiary-care hospital in Korea in 2004. Susceptibility to daptomycin was tested by the CLSI broth microdilution method using Mueller-Hinton broth (MHB) which was adjusted to contain a final concentration of 50 microgram/mL of ionized calcium (Ca2+). Susceptibilities to ampicillin, oxacillin, levofloxacin, vancomycin, and linezolid were tested by the CLSI agar dilution method.
Results: All isolates of methicillin-resistant S. aureus and coagulase-negative staphylococci were inhibited by 1 microgram/mL of daptomycin, and MIC90s were 1 microgram/mL, which were similar to those of vancomycin and linezolid. MIC90s of daptomycin for vancomycin-resistant E. faecalis and E. faecium were 0.5 microgram/mL and 2 microgram/mL, respectively, and all isolates were susceptible to daptomycin. MIC90s of linezolid and levofloxacin for vancomycin-resistant enterococci were 1-2 microgram/mL and 64 microgram/mL, respectively. Resistance rates of vancomycin-resistant E. faecalis and E. faecium to levofloxacin were 100% and 96%, respectively. Daptomycin MICs in MHB supplemented to 20-25 microgram/ml of Ca2+ were 2-8 fold higher than those in MHB supplemented to 50 microgram/mL of Ca2+.
Conclusion: Daptomycin is very active in vitro against methicillin-resistant staphylococci and vancomycin-resistant enterococci isolated in Korea, and it is important to test in vitro activity of daptomycin using MHB containing 50 microgram/mL of Ca2+.
|
|
KEYWORD
|
|
Daptomycin, MRSA, VRE, Staphylococci, Enterococci
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|